The Rig-I/DDX58 Monoclonal Antibody (CAB22478) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, produced through hybridoma technology, is highly specific to human samples and has been validated for use in various applications, including immunofluorescence and flow cytometry.RIG-I/DDX58 is a cytosolic RNA helicase that recognizes viral RNA and triggers the production of type I interferons, leading to the activation of antiviral immune responses. Dysfunction in the RIG-I/DDX58 pathway has been linked to increased susceptibility to viral infections and the development of autoimmune diseases.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Rig-I/DDX58 Monoclonal Antibody
SKU:
CAB22478
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC54110
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of the antiviral innate immune response. Mutations in this gene are associated with Singleton-Merten syndrome 2.
Purification Method
Affinity purification
Gene ID
23586
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Rig-I/DDX58 Rabbit mAb (CAB22478) at 1:2000 dilution. THP-1 cells were treated with LPS (1 μg/ml) at 37℃ for 6 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Western blot analysis of lysates from RAW264.7 cells, using Rig-I/DDX58 Rabbit mAb (CAB22478) at 1:2000 dilution. Raw264. 7 cells were treated with LPS (1 μg/ml) at 37℃ for 8 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
