The ROCK1 Monoclonal Antibody (CAB11158) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced through monoclonal antibody technology, demonstrates high specificity and sensitivity in detecting ROCK1 protein in a variety of sample types, including human tissues and cell lines.ROCK1 is a key regulator of actin cytoskeleton organization and cell adhesion, making it a crucial player in cell motility and tissue morphogenesis. Dysregulation of ROCK1 has been linked to cancer metastasis, cardiovascular diseases, and neurological disorders, making it a promising therapeutic target for drug development.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ROCK1 Monoclonal Antibody
SKU:
CAB11158
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2371
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18.
Purification Method
Affinity purification
Gene ID
6093
RRID
AB_2758435
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ROCK1 Rabbit mAb (CAB11158) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
