The Secretagogin (SCGN) Antibody (CAB12897) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this antibody is highly specific and reactive with human SCGN samples, making it ideal for use in various research applications, including Western blotting.SCGN plays a crucial role in regulating insulin secretion in pancreatic beta cells and protecting neurons from oxidative stress and excitotoxicity. Dysfunction or dysregulation of SCGN has been implicated in various diseases, including diabetes, neurodegenerative disorders, and cancer.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Secretagogin (SCGN) Antibody
SKU:
CAB12897
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The encoded protein is a secreted calcium-binding protein which is found in the cytoplasm. It is related to calbindin D-28K and calretinin. This protein is thought to be involved in KCL-stimulated calcium flux and cell proliferation.
Purification Method
Affinity purification
Gene ID
10590
RRID
AB_2759741
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Secretagogin (SCGN) Rabbit pAb (CAB12897) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Secretagogin (Secretagogin (SCGN)) Rabbit pAb (CAB12897) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of L929 cells using Secretagogin (SCGN) Rabbit pAb (CAB12897) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
