The SCLY Antibody (CAB15842) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high reactivity with human samples and is validated for use in various applications, including Western blotting. By targeting the SCLY protein, this antibody enables precise detection and analysis in different cell types, making it an ideal choice for investigating pathways related to coenzyme Q10 biosynthesis.SCLY plays a vital role in cellular energy production and oxidative stress defense, making it a potential target for research on metabolic disorders, neurodegenerative diseases, and mitochondrial dysfunction.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SCLY Antibody
SKU:
CAB15842
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SCL, hSCL, SCLY
Positive Sample:
U-87MG, Mouse liver
Cellular Localization:
Cytoplasm, Cytosol.
Calculated MW:
48kDa
Observed MW:
48kDa
Selenocysteine lyase (SCLY; EC 4.4.1.16) catalyzes the pyridoxal 5-prime phosphate-dependent conversion of L-selenocysteine to L-alanine and elemental selenium (Mihara et al., 2000 [PubMed 10692412]).
Purification Method
Affinity purification
Gene ID
51540
RRID
AB_2763268
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SCLY Rabbit pAb (CAB15842) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
