The SEMA4C Antibody (CAB8225) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and reactivity with human samples, making it an essential tool for Western blot applications and immunohistochemistry studies.Semaphorin 4C is involved in various biological processes, including cell migration, angiogenesis, and immune response modulation. Its aberrant expression has been linked to diseases such as cancer, autoimmune disorders, and neurological conditions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
SEMA4C Antibody
SKU:
CAB8225
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
HARC QPGG GPPS PPPG IPGQ PLPS PTRL HLGG GRNS NANG YVRL QLGG EDRG GLGH PLPE LADE LRRK LQQR QPLP DSNP EESS V
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SEMAF, SEMAI, SEMACL1, M-SEMA-F, SEMA4C
Positive Sample:
MCF7, BT-474, Mouse heart
Cellular Localization:
Cell Junction, Cytoplasmic Vesicle, Single-Pass Type I Membrane Protein, Postsynaptic Cell Membrane, Postsynaptic Density, Secretory Vesicle, Synapse, Synaptic Vesicle Membrane.
Calculated MW:
93kDa
Observed MW:
110kDa
Predicted to enable chemorepellent activity and semaphorin receptor binding activity. Involved in muscle cell differentiation and positive regulation of stress-activated MAPK cascade. Located in extracellular space.
Purification Method
Affinity purification
Gene ID
54910
RRID
AB_2772177
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SEMA4C Rabbit pAb (CAB8225) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
