The Sestrin 2 Monoclonal Antibody (CAB23685) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using hybridoma technology, has high specificity for detecting Sestrin-2 in various biological samples and is validated for use in techniques such as Western blotting and immunohistochemistry.Sestrin-2 is known for its role in promoting cell survival and reducing oxidative stress, making it a promising target for investigating diseases related to metabolism, aging, and cancer.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Sestrin 2 Monoclonal Antibody
SKU:
CAB23685
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC61316
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HI95, SES2, SEST2, Sestrin 2
Positive Sample:
HeLa, Raw264.7, NIH-3T3
Cellular Localization:
Cytoplasm.
Calculated MW:
54kDa
Observed MW:
60kDa
This gene encodes a member of the sestrin family of PA26-related proteins. The encoded protein may function in the regulation of cell growth and survival. This protein may be involved in cellular response to different stress conditions.
Purification Method
Affinity purification
Gene ID
83667
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using Sestrin 2 Rabbit mAb (CAB23685) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Western blot analysis of various lysates, using Sestrin 2 Rabbit mAb (CAB23685) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
