The SHMT1 Polyclonal Antibody (CAB7727) is a valuable tool for research involving the serine hydroxymethyltransferase 1 (SHMT1) protein. SHMT1 is an enzyme that plays a key role in one-carbon metabolism, a critical pathway for cell growth and proliferation. The antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.By targeting the SHMT1 protein, this antibody allows for the detection and analysis of SHMT1 levels in various cell types, making it an ideal choice for studies in cancer research and metabolism.
Understanding the role of SHMT1 in one-carbon metabolism is essential for developing targeted therapies for diseases such as cancer, where dysregulation of this pathway is common.Overall, the SHMT1 Polyclonal Antibody is a valuable tool for researchers looking to investigate the function and regulation of the SHMT1 enzyme in various biological processes, particularly in the context of cancer and metabolic diseases.
Product Name:
SHMT1 Rabbit Polyclonal Antibody
SKU:
CAB7727
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 285-444 of human SHMT1 (NP_683718.1).
This gene encodes the cytosolic form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one-carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. A pseudogene of this gene is located on the short arm of chromosome 1. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
6470
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using SHMT1 Rabbit pAb (CAB7727) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
