The Siglec1 Polyclonal Antibody (CAB22677) is a high-quality antibody developed for reliable detection and analysis of target proteins. Predicted to enable virion binding activity. Acts upstream of or within positive regulation of T cell apoptotic process and positive regulation of extrinsic apoptotic signaling pathway. Predicted to be located in extracellular region and membrane. Predicted to be integral component of membrane. Predicted to be active in early endosome; late endosome; and plasma membrane. Is expressed in immune system; reproductive system; and skin. Orthologous to human SIGLEC1 (sialic acid binding Ig like lectin 1). RRID Gene ID 20612 Swiss Prot Synonym Sn; Cd169; Siglec-1; Siglec1
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Rat samples.
Product Name:
Siglec1 Polyclonal Antibody
SKU:
CAB22677
Size:
100μL, 20μL
Reactivity:
Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Sn, Cd169, Siglec-1, Siglec1
Positive Sample:
Rat spleen
Cellular Localization:
Early Endosome, Extracellular Region, Late Endosome, Plasma Membrane.
Calculated MW:
183kDa
Observed MW:
200kDa
Predicted to enable virion binding activity. Acts upstream of or within positive regulation of T cell apoptotic process and positive regulation of extrinsic apoptotic signaling pathway. Predicted to be located in extracellular region and membrane. Predicted to be integral component of membrane. Predicted to be active in early endosome; late endosome; and plasma membrane. Is expressed in immune system; reproductive system; and skin. Orthologous to human SIGLEC1 (sialic acid binding Ig like lectin 1). RRID Gene ID 20612 Swiss Prot Synonym Sn; Cd169; Siglec-1; Siglec1
Purification Method:
Affinity purification
Gene ID:
20612
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat spleen using Siglec1 Rabbit pAb (CAB22677) at 1:700 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
