The SLC25A27 Polyclonal Antibody (CAB13834) is a valuable tool for researchers studying SLC25A27, a mitochondrial protein involved in cellular metabolism and energy production. This antibody, generated in rabbits, exhibits high specificity towards human samples and is validated for use in Western blotting applications.SLC25A27, a member of the solute carrier family, plays a crucial role in transporting metabolites across the mitochondrial membrane, contributing to various metabolic pathways crucial for cell survival and function.
Dysregulation of SLC25A27 has been associated with metabolic disorders, neurodegenerative diseases, and cancer, highlighting its potential as a therapeutic target.By enabling the detection and analysis of SLC25A27 protein expression in different cell types, this polyclonal antibody facilitates research in the fields of metabolism, mitochondrial function, and disease pathology. Understanding the function and regulation of SLC25A27 could lead to the development of novel treatments for a variety of human diseases.
Product Name:
SLC25A27 Rabbit Polyclonal Antibody
SKU:
CAB13834
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human SLC25A27 (NP_001190981.1).
Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. Transcripts of this gene are only detected in brain tissue and are specifically modulated by various environmental conditions. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
9481
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from mouse brain, using SLC25A27 Rabbit pAb (CAB13834) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
