The SMARCC1/BAF155 Monoclonal Antibody (CAB4275) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors.
This antibody is validated for use in WB, IF/ICC, ChIP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
SMARCC1/BAF155 Monoclonal Antibody
SKU:
CAB4275
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Clone Number:
ARC0948
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCChIPELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
ChIP
5μg antibody for 10μg-15μg of Chromatin
Synonyms:
HYC5, Rsc8, SRG3, SWI3, BAF155, CRACC1, 55
Positive Sample:
HeLa, HepG2, Mouse brain, Mouse testis, 293T
Cellular Localization:
Nucleus.
Calculated MW:
123kDa
Observed MW:
155kDa/
The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors.
Purification Method
Affinity purification
Gene ID
6599
RRID
AB_2863223
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SMARCC1/BAF155 Rabbit mAb (CAB4275) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
Western blot analysis of lysates from wild type (WT) and SMARCC1/BAF155 knockout (KO) 293T cells using [KO Validated] SMARCC1/BAF155 Rabbit mAb (CAB4275) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 30s.
Chromatin immunoprecipitation analysis of extracts of MCF7 beta-estradiol cells, using SMARCC1/BAF155 antibody (CAB4275) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
