The SMTN Antibody (CAB6745) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for use in immunohistochemistry and Western blot applications.Smoothelin is a key marker for smooth muscle cells and is important in studying various physiological processes such as vascular function and gastrointestinal motility. The Smoothelin Polyclonal Antibody binds specifically to Smoothelin, enabling precise detection and analysis in a variety of tissue types.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SMTN Antibody
SKU:
CAB6745
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SMTN
Positive Sample:
MCF7, 5637, Mouse kidney, Rat lung
Cellular Localization:
Cytoplasm, Cytoskeleton.
Calculated MW:
99kDa
Observed MW:
117kDa
This gene encodes a structural protein that is found exclusively in contractile smooth muscle cells. It associates with stress fibers and constitutes part of the cytoskeleton. This gene is localized to chromosome 22q12.3, distal to the TUPLE1 locus and outside the DiGeorge syndrome deletion. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.
Purification Method
Affinity purification
Gene ID
6525
RRID
AB_2767329
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SMTN Rabbit pAb (CAB6745) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 5min.
