The SOCS2 Monoclonal Antibody (CAB9190) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, generated in rabbits, is highly specific for SOCS2 and is validated for use in various applications, including Western blotting and immunohistochemistry.SOCS2 is a key regulator of cytokine signaling pathways, playing a crucial role in immune response, inflammation, and development. Dysregulation of SOCS2 has been implicated in various diseases, including cancer, autoimmune disorders, and metabolic conditions.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
SOCS2 Monoclonal Antibody
SKU:
CAB9190
Size:
20μL, 100μL
Reactivity:
Human, Rat
Clone Number:
ARC1470
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:1000 - 1:4000
IHC-P
1:100 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CIS2, SSI2, Cish2, SSI-2, SOCS-2, STATI2, SOCS2
Positive Sample:
K-562
Cellular Localization:
Cytoplasm, Cytosol.
Calculated MW:
22kDa
Observed MW:
22kDa
This gene encodes a member of the suppressor of cytokine signaling (SOCS) family. SOCS family members are cytokine-inducible negative regulators of cytokine receptor signaling via the Janus kinase/signal transducer and activation of transcription pathway (the JAK/STAT pathway). SOCS family proteins interact with major molecules of signaling complexes to block further signal transduction, in part, by proteasomal depletion of receptors or signal-transducing proteins via ubiquitination. The expression of this gene can be induced by a subset of cytokines, including erythropoietin, GM-CSF, IL10, interferon (IFN)-gamma and by cytokine receptors such as growth horomone receptor. The protein encoded by this gene interacts with the cytoplasmic domain of insulin-like growth factor-1 receptor (IGF1R) and is thought to be involved in the regulation of IGF1R mediated cell signaling. This gene has pseudogenes on chromosomes 20 and 22. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
8835
RRID
AB_2863683
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from K-562 cells, using SOCS2 Rabbit mAb (CAB9190) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Rat lung using SOCS2 Rabbit mAb (CAB9190) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
