The SPATA18 Antibody (PAC020590) is a reliable tool for researchers studying SPATA18, a key protein involved in spermatogenesis and male fertility. This polyclonal antibody, generated in rabbits, demonstrates high specificity and sensitivity when detecting SPATA18 in human samples. Validated for use in Western blot applications, this antibody binds to the SPATA18 protein, enabling accurate detection and analysis in a variety of cell types.
SPATA18 is essential for sperm development and plays a crucial role in male reproductive function. Research on SPATA18 is important for understanding infertility and reproductive disorders, as well as potential targets for male contraception. This antibody provides a valuable tool for investigating the function and regulation of SPATA18 in the context of reproductive biology and fertility research.
Gel: 8%SDS-PAGE,Lysate: 40 μgPrimary antibody: PACO20590(SPATA18 Antibody) at dilution 1/250 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 5 minutes.
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using PACO20590(SPATA18 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
SPATA18 (spermatogenesis associated 18), also known as Mieap or SPETEX1, is a 538 amino acid, protein that is thought to play a role in cell differentiation during spermatogenesis, particularly during development from late elongate spematids to mature spermatozoa. Localizing to cytoplasm, SPATA18 is encoded by a gene that maps to human chromosome 4q12.
Synonyms:
spermatogenesis associated 18
UniProt Protein Function:
SPATA18: Key regulator of mitochondrial quality that mediates the repairing or degradation of unhealthy mitochondria in response to mitochondrial damage. Mediator of mitochondrial protein catabolic process (also named MALM) by mediating the degradation of damaged proteins inside mitochondria by promoting the accumulation in the mitochondrial matrix of hydrolases that are characteristic of the lysosomal lumen. Also involved in mitochondrion degradation of damaged mitochondria by promoting the formation of vacuole-like structures (named MIV), which engulf and degrade unhealthy mitochondria by accumulating lysosomes. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Belongs to the MIEAP family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: MitochondrialChromosomal Location of Human Ortholog: 4q12Cellular Component: mitochondrial outer membrane; intracellular membrane-bound organelle; cytoplasmMolecular Function: protein bindingBiological Process: response to DNA damage stimulus
