The SPC24 Antibody (CAB16601) is a high-quality antibody developed for reliable detection and analysis of target proteins. Predicted to be involved in cell division. Located in nucleolus and nucleoplasm. Part of Ndc80 complex.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
SPC24 Antibody
SKU:
CAB16601
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SPBC24, SPC24
Positive Sample:
HeLa
Cellular Localization:
Chromosome, Nucleus, Centromere, Kinetochore.
Calculated MW:
22kDa
Observed MW:
26kDa
Predicted to be involved in cell division. Located in nucleolus and nucleoplasm. Part of Ndc80 complex.
Purification Method
Affinity purification
Gene ID
147841
RRID
AB_2772363
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using SPC24 Rabbit pAb (CAB16601) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
