The SPC25 Antibody (CAB10653) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting SPC25 in human samples, making it suitable for use in Western blot applications.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
SPC25 Antibody
SKU:
CAB10653
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AD024, SPBC25, hSpc25, SPC25
Positive Sample:
HeLa, PC-3
Cellular Localization:
Chromosome, Nucleus, Centromere, Kinetochore.
Calculated MW:
26kDa
Observed MW:
26kDa
This gene encodes a protein that may be involved in kinetochore-microtubule interaction and spindle checkpoint activity.
Purification Method
Affinity purification
Gene ID
57405
RRID
AB_2772364
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SPC25 Rabbit pAb (CAB10653) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
