The SPDL1 Polyclonal Antibody (PAC002989) is a valuable tool for researchers studying SPDL1, a protein involved in spindle assembly and chromosome segregation during cell division. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. It targets the SPDL1 protein, enabling precise detection and analysis in a variety of cell types, making it ideal for research in cell biology and cancer biology.
SPDL1 plays a crucial role in ensuring accurate chromosome segregation during cell division, making it essential for the maintenance of genomic stability. Dysregulation of SPDL1 has been implicated in cancer development and progression, making it a potential target for cancer therapy. By studying the function of SPDL1, researchers can gain valuable insights into the mechanisms of cell division and potential therapeutic targets for cancer treatment.
Antibody Name:
SPDL1 Antibody
Antibody SKU:
PACO02989
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
WB:1:500-1:2000
Species Reactivity:
Human
Immunogen:
synthesized peptide derived from the C-terminal region of human CCDC99.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Synonyms:
CCDC99; Protein Spindly; hSpindly; Arsenite-related gene 1 protein; Coiled-coil domain-containing protein 99; Rhabdomyosarcoma antigen MU-RMS-40.4A
UniProt Protein Function:
CCDC99: Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment. Belongs to the Spindly family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Unknown functionChromosomal Location of Human Ortholog: 5q35.1Cellular Component: cytosol; nucleus; outer kinetochore of condensed chromosome; spindle poleMolecular Function: enzyme binding; kinetochore binding; protein bindingBiological Process: establishment of mitotic spindle orientation; mitotic metaphase plate congression; sister chromatid cohesion
UniProt Protein Details:
NCBI Summary:
This gene encodes a coiled-coil domain-containing protein that functions in mitotic spindle formation and chromosome segregation. The encoded protein plays a role in coordinating microtubule attachment by promoting recruitment of dynein proteins, and in mitotic checkpoint signaling. [provided by RefSeq, Jul 2016]
