Description
STAT1 (Phospho-Ser727)Transcription Factor Activity Assay
The STAT1 Phospho-Ser727 Transcription Factor Activity Assay Kit is a highly sensitive and specific assay designed for the accurate detection of STAT1 phosphorylation at serine 727 in cell lysates. This kit allows researchers to analyze the activity of STAT1, a key transcription factor involved in various cellular processes including immune response and cell proliferation.Monitoring the phosphorylation status of STAT1 at serine 727 is critical for understanding its role in signal transduction pathways and gene regulation. Dysregulation of STAT1 activity has been implicated in a variety of diseases, including cancer, autoimmune disorders, and viral infections.
The STAT1 Phospho-Ser727 Transcription Factor Activity Assay Kit provides researchers with a reliable tool for studying these diseases and identifying potential therapeutic targets.With its high sensitivity and specificity, this assay kit delivers accurate and reproducible results, making it suitable for a wide range of research applications. Whether investigating the molecular mechanisms underlying disease pathogenesis or screening potential therapeutic compounds, the STAT1 Phospho-Ser727 Transcription Factor Activity Assay Kit is an essential tool for advancing scientific discovery.
| Product Name: | STAT1 (Phospho-Ser727)Transcription Factor Activity Assay |
| Product Code: | TFAB00056 |
| Target: | STAT1 (Phospho-Ser727) |
| Synonyms: | Transcription factor ISGF-3 components p91/p84 |
| Reactivity: | Human, Mouse, Rat |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie STAT1 (Phospho-Ser727) transcription factor activity assay allows for the detection and qualitative analysis of STAT1 (Phospho-Ser727) active transcription factors in eukaryotic nuclear or cell lysates.
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | STAT1: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of certain receptor tyrosine kinases, GPCRs, and receptors for various interleukins and interferons. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 2q32.2 Cellular Component: axon; cytoplasm; cytosol; dendrite; nuclear chromatin; nucleolus; nucleoplasm; nucleus; perinuclear region of cytoplasm Molecular Function:double-stranded DNA binding; enzyme binding; identical protein binding; protein binding; protein homodimerization activity; signal transducer activity; transcription factor activity; tumor necrosis factor receptor binding Biological Process: apoptosis; blood circulation; defense response to virus; endothelial cell migration; JAK-STAT cascade; negative regulation of angiogenesis; negative regulation of endothelial cell proliferation; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of transcription from RNA polymerase II promoter; negative regulation of viral protein levels in host cell; positive regulation of mesenchymal cell proliferation; positive regulation of smooth muscle cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of apoptosis; regulation of transcription from RNA polymerase II promoter; response to cAMP; response to cytokine stimulus; response to peptide hormone stimulus; transcription, DNA-dependent; tumor necrosis factor-mediated signaling pathway; viral reproduction Disease: Immunodeficiency 31a; Immunodeficiency 31b; Immunodeficiency 31c |
| NCBI Summary: | The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P42224 |
| NCBI GenInfo Identifier: | 2507413 |
| NCBI Gene ID: | 6772 |
| NCBI Accession: | P42224.2 |
| UniProt Secondary Accession: | P42224,Q53S88, Q53XW4, Q68D00, Q9UDL5, A8K989, B2RCA0 D2KFR8, D3DPI7, |
| UniProt Related Accession: | P42224 |
| Molecular Weight: | |
| NCBI Full Name: | Signal transducer and activator of transcription 1-alpha/beta |
| NCBI Synonym Full Names: | signal transducer and activator of transcription 1 |
| NCBI Official Symbol: | STAT1Â Â |
| NCBI Official Synonym Symbols: | CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91Â Â |
| NCBI Protein Information: | signal transducer and activator of transcription 1-alpha/beta |
| UniProt Protein Name: | Signal transducer and activator of transcription 1-alpha/beta |
| UniProt Synonym Protein Names: | Transcription factor ISGF-3 components p91/p84 |
| Protein Family: | Signal transducer and activator of transcription |
| UniProt Gene Name: | STAT1Â Â |
| UniProt Entry Name: | STAT1_HUMAN |
