The STAT4 Monoclonal Antibody (CAB4523) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, produced in rabbits, is highly specific to human samples and suitable for use in Western blot applications. By targeting the STAT4 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an invaluable asset for researchers in fields such as immunology and cancer research.STAT4 is a crucial player in the regulation of immune responses, particularly in the differentiation of T helper 1 cells and the production of pro-inflammatory cytokines.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
STAT4 Monoclonal Antibody
SKU:
CAB4523
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1071
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SLEB11, STAT4
Positive Sample:
HeLa, PC-3, Jurkat
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
86kDa
Observed MW:
86kDa
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. Mutations in this gene may be associated with systemic lupus erythematosus and rheumatoid arthritis. Alternate splicing results in multiple transcript variants that encode the same protein.
Purification Method
Affinity purification
Gene ID
6775
RRID
AB_2863287
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using STAT4 Rabbit mAb (CAB4523) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of C6 cells using STAT4 Rabbit mAb (CAB4523) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
