The STRADA Polyclonal Antibody (CAB21707) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit-derived antibody is highly specific for human samples and is optimized for Western blot applications. By targeting the Strada protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it a valuable asset for research in cancer biology and signal transduction mechanisms.Strada, also known as Ste20-related kinase adaptor alpha, is a key player in the regulation of cell cycle progression and survival signaling.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
STRADA Polyclonal Antibody
SKU:
CAB21707
Size:
20μL, 100μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
LYK5, PMSE, Stlk, STRAD, NY-BR-96, STRAD alpha, STRADA
Positive Sample:
Mouse skeletal muscle
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
48kDa
Observed MW:
Refertofigures
The protein encoded by this gene contains a STE20-like kinase domain, but lacks several residues that are critical for catalytic activity, so it is termed a 'pseudokinase'. The protein forms a heterotrimeric complex with serine/threonine kinase 11 (STK11, also known as LKB1) and the scaffolding protein calcium binding protein 39 (CAB39, also known as MO25). The protein activates STK11 leading to the phosphorylation of both proteins and excluding STK11 from the nucleus. The protein is necessary for STK11-induced G1 cell cycle arrest. A mutation in this gene has been shown to result in polyhydramnios, megalencephaly, and symptomatic epilepsy (PMSE) syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their full-length nature is not known.
Purification Method
Affinity purification
Gene ID
92335
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse skeletal muscle, using STRADA Rabbit pAb (CAB21707) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
