The Syntenin Monoclonal Antibody (CAB5497) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, specifically binds to the syntenin protein in human samples and is validated for use in Western blot applications.Syntenin is a key player in cell biology, interacting with numerous proteins to regulate processes like cell migration and invasion. Its dysregulation has been linked to cancer progression, making it a promising target for cancer research. By using the Syntenin Rabbit Monoclonal Antibody, researchers can detect and analyze syntenin expression in different cell types, providing insights into its role in cancer development and metastasis.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Syntenin Monoclonal Antibody
SKU:
CAB5497
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1410
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
The protein encoded by this gene was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. The syntenin protein contains tandemly repeated PDZ domains that bind the cytoplasmic, C-terminal domains of a variety of transmembrane proteins. This protein may also affect cytoskeletal-membrane organization, cell adhesion, protein trafficking, and the activation of transcription factors. The protein is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus. Alternative splicing results in multiple transcript variants encoding different isoforms. Related pseudogenes have been identified on multiple chromosomes.
Purification Method
Affinity purification
Gene ID
6386
RRID
AB_2863506
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Syntenin Rabbit mAb (CAB5497) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded Rat brain using Syntenin Rabbit mAb (CAB5497) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse brain using Syntenin Rabbit mAb (CAB5497) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Syntenin Rabbit mAb (CAB5497) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
