The Brachyury Antibody (CAB16887) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Variation in this gene was associated with susceptibility to neural tube defects and chordoma. A mutation in this gene was found in a family with sacral agenesis with vertebral anomalies.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
Brachyury Antibody
SKU:
CAB16887
Size:
100μL, 20μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:1000
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
T, TFT, SAVA, Brachyury
Positive Sample:
H460
Cellular Localization:
Nucleus.
Calculated MW:
47kDa
Observed MW:
47kDa
The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Variation in this gene was associated with susceptibility to neural tube defects and chordoma. A mutation in this gene was found in a family with sacral agenesis with vertebral anomalies.
Purification Method
Affinity purification
Gene ID
6862
RRID
AB_2772502
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from H460 cells, using Brachyury Rabbit pAb (CAB16887) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of NCI-H460 cells using Brachyury Rabbit pAb (CAB16887) aBrachyury diluBrachyuryion of 1:200 (40x lens). Blue: DAPI for nuclear sBrachyuryaining.
