The Brachyury Antibody (CAB16887) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific to human samples and is validated for Western blot applications. By targeting the CD300A protein, researchers can effectively detect and analyze its presence in various cell types, making it a versatile tool for studies in immunology and cancer research.CD300A, also referred to as an immune inhibitory receptor, is essential for maintaining immune homeostasis by controlling inflammation and suppressing allergic reactions.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
Brachyury Antibody
SKU:
CAB16887
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
T, TFT, SAVA, Brachyury
Positive Sample:
H460
Cellular Localization:
Nucleus.
Calculated MW:
47kDa
Observed MW:
47kDa
The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Variation in this gene was associated with susceptibility to neural tube defects and chordoma. A mutation in this gene was found in a family with sacral agenesis with vertebral anomalies.
Purification Method
Affinity purification
Gene ID
6862
RRID
AB_2772502
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from H460 cells, using Brachyury Rabbit pAb (CAB16887) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of NCI-H460 cells using Brachyury Rabbit pAb (CAB16887) aBrachyury diluBrachyuryion of 1:200 (40x lens). Blue: DAPI for nuclear sBrachyuryaining.
