The TEAD1 Monoclonal Antibody (CAB5218) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed and validated for use in various research applications, specifically recognizes TEAD1 in human samples with high specificity and sensitivity.TEAD1 is a critical player in the Hippo signaling pathway, which controls organ size, tissue regeneration, and tumorigenesis. Dysregulation of TEAD1 has been linked to various diseases, including cancer and developmental disorders.
This antibody is validated for use in WB, IP, ChIP, ELISA, CUT&Tag applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TEAD1 Monoclonal Antibody
SKU:
CAB5218
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1157
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
HeLa, Mouse skeletal muscle, Mouse heart, Rat skeletal muscle, Rat heart
Cellular Localization:
Nucleus.
Calculated MW:
48kDa
Observed MW:
50kDa
This gene encodes a ubiquitous transcriptional enhancer factor that is a member of the TEA/ATTS domain family. This protein directs the transactivation of a wide variety of genes and, in placental cells, also acts as a transcriptional repressor. Mutations in this gene cause Sveinsson's chorioretinal atrophy. Additional transcript variants have been described but their full-length natures have not been experimentally verified.
Purification Method
Affinity purification
Gene ID
7003
RRID
AB_2863490
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using TEAD1 Rabbit mAb (CAB5218) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunoprecipitation analysis of 600 μg extracts of Mouse skeletal muscle cells using 3 μg TEAD1 antibody (CAB5218). Western blot was performed from the immunoprecipitate using TEAD1 antibody (CAB5218) at a dilution of 1:1000.
CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina(RK20265) from 10⁵ Hep G2 cells with 1 μg TEAD1 Rabbit mAb (CAB5218), along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of TEAD1 in representative gene loci (SLC7A5), as shown in figure.
