The Thymidine Kinase 1 Monoclonal Antibody (CAB13612) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a cytosolic enzyme that catalyzes the addition of a gamma-phosphate group to thymidine. This creates dTMP and is the first step in the biosynthesis of dTTP, which is one component required for DNA replication. The encoded protein, whose levels fluctuate depending on the cell cycle stage, can act as a low activity dimer or a high activity tetramer. High levels of this protein have been used as a biomarker for diagnosing and categorizing many types of cancers. RRID AB_2861691 Gene ID 7083 Swiss Prot Synonym TK2; Thymidine Kinase 1
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Thymidine Kinase 1 Monoclonal Antibody
SKU:
CAB13612
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
ARC0719
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TK2, Thymidine Kinase 1
Positive Sample:
HeLa, 293T, Jurkat
Cellular Localization:
Cytoplasm.
Calculated MW:
25kDa
Observed MW:
26kDa
The protein encoded by this gene is a cytosolic enzyme that catalyzes the addition of a gamma-phosphate group to thymidine. This creates dTMP and is the first step in the biosynthesis of dTTP, which is one component required for DNA replication. The encoded protein, whose levels fluctuate depending on the cell cycle stage, can act as a low activity dimer or a high activity tetramer. High levels of this protein have been used as a biomarker for diagnosing and categorizing many types of cancers. RRID AB_2861691 Gene ID 7083 Swiss Prot Synonym TK2; Thymidine Kinase 1
Purification Method:
Affinity purification
Gene ID:
7083
RRID:
AB_2861691
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Thymidine Kinase 1 Rabbit mAb (CAB13612) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
