The TMPRSS6 Polyclonal Antibody (CAB10642) is a high-quality antibody developed for reliable detection and analysis of target proteins. TMPRSS6 is involved in iron homeostasis and regulation of hepcidin, making it a key player in iron metabolism and related disorders such as iron-deficiency anemia. This antibody, raised in rabbits, is highly specific to TMPRSS6 and is validated for use in various applications, including Western blot and immunohistochemistry.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TMPRSS6 Polyclonal Antibody
SKU:
CAB10642
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MT2, IRIDA, TMPRSS6
Positive Sample:
LO2, Mouse liver, Rat liver
Cellular Localization:
Cell Membrane, Single-Pass Type Ii Membrane Protein.
Calculated MW:
90kDa
Observed MW:
110kDa
The protein encoded by this gene is a type II transmembrane serine proteinase that is found attached to the cell surface. The encoded protein may be involved in matrix remodeling processes in the liver. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
164656
RRID
AB_2772648
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using TMPRSS6 Rabbit pAb (CAB10642) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of lysates from Rat liver, using TMPRSS6 Rabbit pAb (CAB10642) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
