The TOLLIP Antibody (CAB2202) is a high-quality antibody developed for reliable detection and analysis of target proteins. Tollip is known to interact with various proteins involved in innate immune responses, making it a key player in the immune system's defense against pathogens.This polyclonal antibody is produced in rabbits and has been validated for use in Western blot applications. It specifically binds to Tollip protein, allowing for detection and analysis in a variety of cell types. Researchers studying immunology, infectious diseases, and inflammatory conditions will find this antibody essential for their investigations.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TOLLIP Antibody
SKU:
CAB2202
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
IL-1RAcPIP, TOLLIP
Positive Sample:
SW620, HeLa, 293T, Mouse testis, Mouse brain, Mouse thymus, Rat testis, Rat liver
Cellular Localization:
Cytoplasm.
Calculated MW:
30kDa
Observed MW:
30kDa
This gene encodes a ubiquitin-binding protein that interacts with several Toll-like receptor (TLR) signaling cascade components. The encoded protein regulates inflammatory signaling and is involved in interleukin-1 receptor trafficking and in the turnover of IL1R-associated kinase. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
54472
RRID
AB_2764219
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% Thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using TOLLIP Rabbit pAb (CAB2202) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Rat brain using TOLLIP Rabbit pAb (CAB2202) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
