Total Protein Assay Kit - Information
Assay Genie's fluorometric protein assay kit is based on an improved o-phthalaldehyde method. This reagent reacts with primary amines in protein or peptide and forms a blue fluorescent product, allowing detection of nanograms of proteins. The fluorescence intensity (Ex/Em = 360/450nm) is proportional to the protein concentration in the sample.
For quantitative determination of total protein
Total Protein Assay Kit - Key Features
- Fast and sensitive. Assay is completed within a few minutes. Linear detection range of 0.05 - 200 ug/mL BSA.
- Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Total Protein Assay Kit - Data Sheet
|Kit Includes||Reagent: 20 mL Standard: 1 mL 1mg/mL BSA|
|Kit Requires||Pipetting devices, centrifuge tubes, black flat bottom 96-well plates and plate reader|
|Method of Detection||FL360/450nm|
|Detection Limit||50 ng/mL|
|Protocol Length||10 min|
|Storage||For long-term storage, keep kit at -20°C|
|Shelf Life||6 months|
The protein or peptide is known as the "building blocks of life" and is one of the most important macromolecules in life science. Protein determination is a very common practice. Simple, direct and automation-ready procedures for measuring protein or peptide concentration are very desirable.