Assay Genie's Total Protein Assay Kit is based on an improved pyrogallol red-molybdate protein dye-binding assay. The color intensity at 600nm is directly proportional to the total protein concentration in the sample. This single reagent can be used for the quantitative determination of total protein in urine (proteinuria) and cerebrospinal fluid (blood brain barrier).
ApplicationsFor quantitative determination of total protein.
- Fast and sensitive. Assay takes 10 min. Linear detection range of 5 - 200 mg/dL protein.
- Robust and Reliable. Substances in urine have minimal interference (< 2%).
- Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
|Kit Includes||Reagent 20 mL, Standard 1 mL 100 mg/dL|
|Kit Requires||For 96-well plate assays: pipetting devices, clear flat bottom 96-well plates, and plate reader. For cuvette assays: pipetting devices, cuvettes and spectrophotometer capable of reading OD600nm.|
|Method of Detection||OD600nm (Pyrogallol Red)|
|Detection Limit||5 mg/dL|
|Samples||Urine, cerebrospinal fluid etc|
|Protocol Length||10 min|
|Storage||Store kit at 2-8°C.|
|Shelf Life||6 months|