The U2AF1 Polyclonal Antibody (CAB1046) is a valuable tool for researchers studying U2AF1, a key protein involved in pre-mRNA splicing. This antibody, produced in rabbits, is highly specific to human samples and is optimized for use in Western blot applications. By targeting the U2AF1 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential tool for studies in molecular biology and RNA processing.U2AF1 is a critical component of the spliceosome complex, playing a crucial role in mRNA processing and gene expression regulation.
Mutations in U2AF1 have been linked to various diseases, including cancer and developmental disorders, underscoring the importance of understanding its function. By using the U2AF1 Polyclonal Antibody, researchers can delve into the intricacies of RNA splicing and its implications in disease pathogenesis, paving the way for potential therapeutic interventions.
Product Name:
U2AF1 Rabbit Polyclonal Antibody
SKU:
CAB1046
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human U2AF1 (NP_006749.1).
This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified.
Purification Method:
Affinity purification
Gene ID:
7307
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using U2AF1 Rabbit pAb (CAB1046) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
