The UBE2J2 Antibody (CAB7316) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the UBE2J2 protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for investigations in the fields of molecular biology and cell signaling.UBE2J2 is involved in protein degradation pathways by catalyzing the attachment of ubiquitin molecules to target proteins, marking them for degradation by the proteasome. Dysregulation of ubiquitination processes has been implicated in various diseases, including cancer and neurodegenerative disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
UBE2J2 Antibody
SKU:
CAB7316
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
UBC6, NCUBE2, NCUBE-2, PRO2121, UBE2J2
Positive Sample:
U-251MG, HeLa, 293T, Mouse testis, Mouse brain
Cellular Localization:
Endoplasmic Reticulum Membrane, Single-Pass Type Iv Membrane Protein.
Calculated MW:
29kDa
Observed MW:
29kDa
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.
Purification Method
Affinity purification
Gene ID
118424
RRID
AB_2767856
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using UBE2J2 Rabbit pAb (CAB7316) at 1:1000 dilution._Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 90s.
