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Anti-UBE2K Antibody (CAB1086)

SKU:
CAB1086
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction
$167 - $503
Frequently bought together:

Description

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Antibody Name:Anti-UBE2K Antibody
Antibody SKU:CAB1086
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UBE2K (NP_005330.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:22Rv1, SKOV3, SW480, HL-60, Raji, U-251MG, MCF7
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UBE2K (NP_005330.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MANI AVQR IKRE FKEV LKSE ETSK NQIK VDLV DENF TELR GEIA GPPD TPYE GGRY QLEI KIPE TYPF NPPK VRFI TKIW HPNI SSVT GAIC LDIL KDQW
Gene ID:3093
Uniprot:P61086
Cellular Location:Cytoplasm
Calculated MW:16kDa/17kDa/22kDa
Observed MW:22kDa
Synonyms:UBE2K, E2-25K, HIP2, HYPG, LIG, UBC1
Background:The protein encoded by this gene belongs to the ubiquitin-conjugating enzyme family. This protein interacts with RING finger proteins, and it can ubiquitinate huntingtin, the gene product for Huntington's disease. Known functions for this protein include a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases, a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation. Multiple transcript variants encoding different isoforms have been identified for this gene.
UniProt Protein Function:UBE2K: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein- dependent degradation of RB1. Interacts with RNF138/NARF. Interacts with BRCA1. By aggregated low-density lipoprotein. Expressed in all tissues tested, including spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, T-lymphocytes, monocytes, granulocytes and bone marrow mononuclear cells. Highly expressed in brain, with highest levels found in cortex and striatum and at lower levels in cerebellum and brainstem. Belongs to the ubiquitin-conjugating enzyme family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ubiquitin ligase; Ligase; Ubiquitin conjugating system; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 4p14

Cellular Component: intermediate filament cytoskeleton; cytoplasm; nucleus; actin cytoskeleton

Molecular Function:protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; regulation of proteasomal ubiquitin-dependent protein catabolic process

NCBI Summary:The protein encoded by this gene belongs to the ubiquitin-conjugating enzyme family. This protein interacts with RING finger proteins, and it can ubiquitinate huntingtin, the gene product for Huntington's disease. Known functions for this protein include a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases, a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation. Multiple transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P61086
NCBI GenInfo Identifier:46577658
NCBI Gene ID:3093
NCBI Accession:P61086.3
UniProt Related Accession:P61086
Molecular Weight:
NCBI Full Name:Ubiquitin-conjugating enzyme E2 K
NCBI Synonym Full Names:ubiquitin conjugating enzyme E2 K
NCBI Official Symbol:UBE2K  
NCBI Official Synonym Symbols:LIG; HIP2; HYPG; UBC1; E2-25K  
NCBI Protein Information:ubiquitin-conjugating enzyme E2 K
UniProt Protein Name:Ubiquitin-conjugating enzyme E2 K
UniProt Synonym Protein Names:Huntingtin-interacting protein 2; HIP-2; Ubiquitin carrier protein; Ubiquitin-conjugating enzyme E2-25 kDa; Ubiquitin-conjugating enzyme E2(25K); Ubiquitin-conjugating enzyme E2-25K; Ubiquitin-protein ligase
Protein Family:High-potential iron-sulfur protein
UniProt Gene Name:UBE2K  
UniProt Entry Name:UBE2K_HUMAN
Anti-UBE2K Antibody (CAB1086)Western blot analysis of extracts of various cell lines, using UBE2K antibody (CAB1086) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

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