The UCH37/UCHL5 Monoclonal Antibody (CAB23792) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, developed using advanced techniques, offers high specificity and sensitivity for detecting and analyzing these critical proteasome components in various biological samples.UCH37 and UCHL5 are deubiquitinating enzymes involved in the regulation of protein degradation and turnover. Dysregulation of these proteins has been linked to various diseases, including cancer, neurodegenerative disorders, and inflammatory conditions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
UCH37/UCHL5 Monoclonal Antibody
SKU:
CAB23792
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC60845
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Enables endopeptidase inhibitor activity; proteasome binding activity; and thiol-dependent deubiquitinase. Involved in negative regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of smoothened signaling pathway; and protein deubiquitination. Located in cytosol; nucleolus; and nucleoplasm. Colocalizes with Ino80 complex.
Purification Method
Affinity purification
Gene ID
51377
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using UCH37/UCHL5 Rabbit mAb (CAB23792) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
