Description
| Product Name: | Human Cardiac Troponin I (TNNI3/cTn-I) Superset Max DIY ELISA |
| Target: | TNNI3/cTn-I |
| SKU: | AEES00741 |
| Size: | 96T * 5, 96T * 15 |
| Reactivity: | Human |
| Range: | 0.39~25 ng/mL |
| Sample Type: | Serum, Plasma |
| Detection Method: | Sandwich ELISA |
| Assay Time: | 24 hours |
| Sample volume: | 100uL |
| Storage: | -20℃. Please refer to the instructions for details. |
| Component | Specification | Storage |
| Micro ELISA pre-Plate(Dismountable) | 96T*5：5 plates, 96T | -20℃, 12 months |
| Reference Standard | 96T*5：1 vial | -20℃, 12 months |
| Concentrated Capture Ab | 96T*5：1 vial 120μL | -20℃, 12 months |
| Concentrated Biotinylated Detection Ab | 96T*5：1 vial 120μL | -20℃, 12 months |
| Concentrated HRP Conjugate | 96T*5：1 vial 120μL | -20℃(Protect from light), 12 months |
| Product Manual | 1 Copy | - |
| Certificate of Analysis | 1 Copy | - |
Other materials and equipment required:
- Microplate reader with 450 nm wavelength filter
- High-precision transfer pipette, EP tubes and disposable pipette tips
- Incubator capable of maintaining 37℃
- Deionized or distilled water
- Absorbent paper
- Loading slot
| Step 1: | Analyte-specific antibody (capture antibody) is bound to a pre-microplate, unbound capture antibody is washed away. Then plates are blocked. |
| Step 2: | Samples or standards are added and any analyte present is bound by the immobilized capture antibody. Unbound materials are decanted. |
| Step 3: | The added Biotinylated Detection Ab will bind to the analyte been captured by the immobilized antibody. Unbound Biotinylated Detection Ab is washed away. |
| Step 4: | HPR Conjugate is used to bind to the Biotinylated Detection Ab. Unbound HPR Conjugate is washed away. |
| Step 5: | TMB substrate solution is added to the wells and a blue color develops in proportion to the amount of analyte present in the sample. Then the Stop Solution is added to stop the color change of substrate, and the color inside the well changes from blue to yellow. The absorbance of the color at 450 nm is measured. |
| Serum: | Allow samples to clot for 1 hour at room temperature or overnight at 2-8℃before centrifugation for 20 min at 1000×g at 2-8℃. Collect the supernatant to carry out the assay. |
| Plasma: | Collect plasma using EDTA-Na2 as an anticoagulant. Centrifuge samples for 15 min at 1000×g at 2-8℃ within 30 min of collection. Collect the supernatant to carry out the assay. |
