Human Cardiac Troponin I (TNNI3/cTn-I) Superset Max DIY ELISA
The Human Cardiac Troponin I (TNNI3/cTn-I) Superset Max DIY ELISA is a high-performance “do-it-yourself” development ELISA kit, designed for the quantitative measurement of TNNI3/cTn-I. This kit includes all essential components required to optimize & scale up to 5 x 96-well ELISA plates.
SuperSet Max DIY ELISA Kit contains: ✔ Carefully optimized capture and detection antibodies ✔ Purified recombinant protein standard ✔ 5 x 96-well ELISA plates ✔ Streptavidin-HRP Conjugate
With premium reagents and pre-validated components, the SuperSet Max range provides researchers with a reliable, flexible solution for measuring analytes in cell culture supernatants, serum, plasma, and other complex biological samples.
Product Name:
Human Cardiac Troponin I (TNNI3/cTn-I) Superset Max DIY ELISA
SKU:
AEES00741
Size:
96T * 5, 96T * 15
Target:
TNNI3/cTn-I
Reactivity:
Human
Range:
0.39~25 ng/mL
Sample Type:
Serum, Plasma
Detection Method:
Sandwich ELISA
Assay Time:
24 hours
Sample volume:
100uL
Storage:
-20℃. Please refer to the instructions for details.
*Available in an Accessory Pack for ELISA Set, Assay Genie product AEES03043.
Microtitre plate reader with 450nm filter (optional 630nm reference)
Microplate washer or wash bottle
10, 50, 100, 200, 1000µl micropipettes with disposable tips
50-300µl multi-channel micropipette with disposable tips
Multichannel micropipette reagent reservoirs
Distilled water
Vortex mixer
Miscellaneous laboratory plastic/glass (sterile if possible)
Step 1:
Analyte-specific antibody (capture antibody) is bound to a pre-microplate, unbound capture antibody is washed away. Then plates are blocked.
Step 2:
Samples or standards are added and any analyte present is bound by the immobilized capture antibody. Unbound materials are decanted.
Step 3:
The added Biotinylated Detection Ab will bind to the analyte been captured by the immobilized antibody. Unbound Biotinylated Detection Ab is washed away.
Step 4:
HPR Conjugate is used to bind to the Biotinylated Detection Ab. Unbound HPR Conjugate is washed away.
Step 5:
TMB substrate solution is added to the wells and a blue color develops in proportion to the amount of analyte present in the sample. Then the Stop Solution is added to stop the color change of substrate, and the color inside the well changes from blue to yellow. The absorbance of the color at 450 nm is measured.
Serum:
Allow samples to clot for 1 hour at room temperature or overnight at 2-8℃before centrifugation for 20 min at 1000×g at 2-8℃. Collect the supernatant to carry out the assay.
Plasma:
Collect plasma using EDTA-Na2 as an anticoagulant. Centrifuge samples for 15 min at 1000×g at 2-8℃ within 30 min of collection. Collect the supernatant to carry out the assay.
