The WDR61 Antibody (CAB15520) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody has been raised in rabbits and demonstrates high reactivity with human samples, making it suitable for use in Western blot applications. By binding specifically to WDR61, this antibody enables accurate detection and analysis of the protein in a wide range of cell types.WDR61, also known as WD repeat-containing protein 61, plays a crucial role in cell cycle progression and DNA replication, making it a key player in cell growth and proliferation.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
WDR61 Antibody
SKU:
CAB15520
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SKI8, REC14, WDR61
Positive Sample:
HeLa, A-549, mouse stomach, mouse brain, mouse kidney, rat pancreas
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
34kDa
Observed MW:
36kDa
WDR61 is a subunit of the human PAF and SKI complexes, which function in transcriptional regulation and are involved in events downstream of RNA synthesis, such as RNA surveillance (Zhu et al., 2005 [PubMed 16024656]).
Purification Method
Affinity purification
Gene ID
80349
RRID
AB_2762921
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using WDR61 Rabbit pAb (CAB15520) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 40s.
Immunohistochemistry analysis of paraffin-embedded Human placenta using WDR61 Rabbit pAb (CAB15520) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
