The WIF1 Monoclonal Antibody (CAB5171) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting WIF1 in human samples and is validated for use in various applications, including Western blotting.WIF1 is a crucial player in regulating the Wnt signaling pathway, which is involved in various cellular processes such as cell proliferation, differentiation, and migration. Dysregulation of Wnt signaling has been implicated in numerous diseases, including cancer and developmental disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
WIF1 Monoclonal Antibody
SKU:
CAB5171
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1161
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
WIF-1, WIF1
Positive Sample:
HeLa, Mouse lung, Mouse stomach, Rat lung, Rat stomach
Cellular Localization:
Secreted.
Calculated MW:
42kDa
Observed MW:
42kDa
The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. This protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers.
Purification Method
Affinity purification
Gene ID
11197
RRID
AB_2863474
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using WIF1 Rabbit mAb (CAB5171) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
