The 14-3-3 Theta Monoclonal Antibody (CAB8936) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed from rabbit monoclonal sources, demonstrates high specificity and sensitivity in detecting human samples through Western blot analysis.The 14-3-3 Theta protein plays a significant role in cell cycle regulation, apoptosis, and intracellular signaling pathways. Dysregulation of this protein has been linked to various diseases, including cancer, neurodegenerative disorders, and metabolic syndromes. By targeting the 14-3-3 Theta protein with this antibody, researchers can gain insight into its functions and mechanisms in different cell types, aiding in further studies in molecular biology and disease pathology.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
14-3-3 Theta Monoclonal Antibody
SKU:
CAB8936
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1358
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
1C5, HS1, 14-3-3, 14-3-3 Theta
Positive Sample:
C6, U-251MG, Mouse testis, Mouse brain, Rat testis
Cellular Localization:
Cytoplasm.
Calculated MW:
28kDa
Observed MW:
28kDa
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease.
Purification Method
Affinity purification
Gene ID
10971
RRID
AB_2863632
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using 14-3-3 Theta Rabbit mAb (CAB8936) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of NIH-3T3 cells using 14-3-3 Theta Rabbit mAb (CAB8936) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
