The 4-HNE ELISA Kit is designed for the quantitative detection of 4-Hydroxynonenal (4-HNE) levels in various biological samples. 4-HNE is a critical biomarker of oxidative stress and lipid peroxidation, involved in a wide array of cellular activities, including cell proliferation, differentiation, and apoptosis. It plays a significant role in the body's response to oxidative stress and has been implicated in diverse diseases, including cardiac disorders, neurodegenerative diseases, and cancer. Accurate measurement of 4-HNE is essential for understanding its involvement in disease mechanisms and for developing targeted therapeutic strategies. Assay Genie's 4-HNE ELISA Kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under stringent quality control standards, this kit provides robust performance and is easy to use, making it an excellent choice for both research and clinical applications. Trust Assay Genie's 4-HNE ELISA Kit for accurate and dependable quantification of this crucial biomarker in your studies.
Product Name:
4-HNE (4-Hydroxynonenal) ELISA Kit
SKU:
AEES00007
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
