Description
AAK1 Antibody (PACO23021)
The AAK1 Polyclonal Antibody (PAC023021) is a valuable tool for researchers studying AAK1, a key protein involved in various cellular processes including endocytosis, cell signaling, and cell cycle progression. This antibody, produced in rabbits, exhibits strong reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the AAK1 protein, this antibody enables precise detection and analysis in diverse cell types.AAK1, also known as AP2 associated kinase 1, is a critical regulator of clathrin-mediated endocytosis and plays a crucial role in the internalization of a wide range of cell surface receptors and proteins.
Dysregulation of AAK1 has been implicated in various diseases, including cancer, neurodegenerative disorders, and infectious diseases. Therefore, studying the functions and pathways involving AAK1 is essential for gaining insights into disease mechanisms and identifying potential therapeutic targets.The AAK1 Polyclonal Antibody is a valuable tool for researchers interested in exploring the roles of AAK1 in cellular processes and disease pathogenesis. Its versatility and specificity make it an ideal choice for studies in cell biology, signal transduction, and drug discovery efforts targeting AAK1-related pathways.
| Antibody Name: | AAK1 Antibody (PACO23021) |
| Antibody SKU: | PACO23021 |
| Size: | 100ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:1000, IHC:1:50-1:200 |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Fusion protein corresponding to N terminal 236 amino acids of Human Adaptor-associated protein kinase 1 |
| Form: | Liquid |
| Storage Buffer: | Supplied at 1.1mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol. |
| Purification Method: | Affinity purification |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Gel: 10%SDS-PAGE Lysate: 30 ug Human fetal brain tissue lysate Primary antibody: 1/500 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 30 seconds. |
 | The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 31024 (AAK1 Antibody) at dilution 1/30, on the right is treated with the fusion protein. |
| Background: | Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. |
| Synonyms: | Adaptor-associated protein kinase 1, DKFZp686K16132, KIAA1048 |
| UniProt Protein Function: | AAK1: an NAK kinase. Plays a regulatory role in clathrin-mediated endocytosis, including the regulation of fatty acid synthesis and cholesterol synthesis. |
| UniProt Protein Details: | Protein type:Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, Other; Other group; NAK family Chromosomal Location of Human Ortholog: 2p14 Cellular Component: clathrin-coated vesicle; cytoplasm; extrinsic to plasma membrane; leading edge; terminal button Molecular Function:Notch binding; protein binding; protein serine/threonine kinase activity Biological Process: positive regulation of Notch signaling pathway; protein amino acid autophosphorylation; protein amino acid phosphorylation; protein stabilization; regulation of protein localization |
| NCBI Summary: | Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q2M2I8 |
| NCBI GenInfo Identifier: | 300669613 |
| NCBI Gene ID: | 22848 |
| NCBI Accession: | Q2M2I8.3 |
| UniProt Secondary Accession: | Q2M2I8,Q4ZFZ3, Q53RX6, Q9UPV4, |
| UniProt Related Accession: | Q2M2I8 |
| Molecular Weight: | 93,578 Da |
| NCBI Full Name: | AP2-associated protein kinase 1 |
| NCBI Synonym Full Names: | AP2 associated kinase 1 |
| NCBI Official Symbol: | AAK1Â Â |
| NCBI Protein Information: | AP2-associated protein kinase 1 |
| UniProt Protein Name: | AP2-associated protein kinase 1 |
| UniProt Synonym Protein Names: | Adaptor-associated kinase 1 |
| Protein Family: | AP2-associated protein kinase |
| UniProt Gene Name: | AAK1Â Â |
| UniProt Entry Name: | AAK1_HUMAN |
| Antibodies | ELISA Kits |
| AAK1 Antibody (PACO00349) | Human AAK1 (AP2-associated protein kinase 1) ELISA Kit (HUFI05483) |
| AAK1 Colorimetric Cell-Based ELISA |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
