The ABCA1 Monoclonal Antibody (CAB21976) is a valuable tool for researchers studying ABCA1, a critical protein involved in cholesterol transport and lipid metabolism. This monoclonal antibody, generated from mouse hybridoma cells, demonstrates high specificity and sensitivity in detecting ABCA1 in human samples, making it suitable for applications such as Western blotting and immunofluorescence.ABCA1 is a key regulator of cellular cholesterol efflux, playing a crucial role in maintaining cholesterol homeostasis and preventing the development of atherosclerosis. Dysregulation of ABCA1 function has been linked to various metabolic disorders and cardiovascular diseases, emphasizing the importance of studying this protein in research settings.
By using the ABCA1 Monoclonal Antibody (CAB21976), researchers can accurately assess ABCA1 expression levels and localization in different cell types, paving the way for a better understanding of the mechanisms underlying cholesterol metabolism and potential therapeutic interventions targeting ABCA1-related pathways. This antibody is a valuable asset for studies focusing on lipid disorders, cardiovascular health, and metabolic diseases.
Product Name:
ABCA1 Monoclonal Antibody
SKU:
CAB21976
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1170-1350 of human ABCA1 (NP_005493.2).
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesteral efflux pump in the cellular lipid removal pathway. Mutations in both alleles of this gene cause Tangier disease and familial high-density lipoprotein (HDL) deficiency.
Purification Method:
Affinity purification
Gene ID:
19
Clone Number:
ARC54239
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Confocal imaging of PC-12 cells using ABCA1 Rabbit mAb (A21976,dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007,dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.
