Anti-ABCA1 Antibody (CAB16337)

Antibody Name:	Anti-ABCA1 Antibody
Antibody SKU:	CAB16337
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1870-2120 of human ABCA1 (NP_005493.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain, Rat brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1870-2120 of human ABCA1 (NP_005493.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	IQYR FFIR PRPV NAKL SPLN DEDE DVRR ERQR ILDG GGQN DILE IKEL TKIY RRKR KPAV DRIC VGIP PGEC FGLL GVNG AGKS STFK MLTG DTTV TRGD AFLN KNSI LSNI HEVH QNMG YCPQ FDAI TELL TGRE HVEF FALL RGVP EKEV GKVG EWAI RKLG LVKY GEKY AGNY SGGN KRKL STAM ALIG GPPV VFLD EPTT GMDP KARR FLWN CALS VVKE GRSV VLTS HSME ECEA LCTR MAIM VNG
Gene ID:	19
Uniprot:	O95477
Cellular Location:	Membrane, Multi-pass membrane protein
Calculated MW:	254kDa
Observed MW:	270KDa

Synonyms:

ABCA1, ABC-1, ABC1, CERP, HDLDT1, TGD

Background:

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesteral efflux pump in the cellular lipid removal pathway. Mutations in this gene have been associated with Tangier's disease and familial high-density lipoprotein deficiency.

UniProt Code:	O95477
NCBI GenInfo Identifier:	308153644
NCBI Gene ID:	19
NCBI Accession:	O95477.3
UniProt Secondary Accession:	O95477,Q5VX33, Q96S56, Q96T85, Q9NQV4, Q9UN06, Q9UN07 Q9UN08, Q9UN09,
UniProt Related Accession:	O95477
Molecular Weight:	254,302 Da
NCBI Full Name:	ATP-binding cassette sub-family A member 1
NCBI Synonym Full Names:	ATP-binding cassette, sub-family A (ABC1), member 1
NCBI Official Symbol:	ABCA1
NCBI Official Synonym Symbols:	TGD; ABC1; CERP; ABC-1; HDLDT1
NCBI Protein Information:	ATP-binding cassette sub-family A member 1; membrane-bound; ATP-binding cassette transporter A1; cholesterol efflux regulatory protein
UniProt Protein Name:	ATP-binding cassette sub-family A member 1
UniProt Synonym Protein Names:	ATP-binding cassette transporter 1; ABC-1; ATP-binding cassette 1; Cholesterol efflux regulatory protein
Protein Family:	ABC transporter A family
UniProt Gene Name:	ABCA1
UniProt Entry Name:	ABCA1_HUMAN

	Western blot analysis of extracts of Rat brain, using ABCA1 Polyclonal Antibody (CAB16337) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
	Western blot analysis of extracts of various cell lines, using ABCA1 Polyclonal Antibody (CAB16337) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse kidney using ABCA1 Rabbit pAb (CAB16337) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat ovary using ABCA1 Rabbit pAb (CAB16337) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using ABCA1 Rabbit pAb (CAB16337) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-ABCA1 Antibody (CAB7228)	Human ABCA1 / ATP-binding cassette sub-family A member 1 ELISA Kit
	Human ABCA1(ATP-binding cassette transporter A1) CLIA Kit (HUES00371)
	Human ABCA1(ATP Binding Cassette Transporter A1) ELISA Kit (HUES01725)
	Human ATP-binding cassette sub-family A member 1 (ABCA1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)