The MRP1/ABCC1 Antibody (CAB3027) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, shows strong reactivity with human samples and has been validated for use in Western blot applications.ABCC1, also known as multidrug resistance protein 1 (MDR1), is involved in drug resistance and transport across cell membranes. It is particularly important in cancer research, as overexpression of ABCC1 is associated with resistance to chemotherapy drugs.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MRP1/ABCC1 Antibody
SKU:
CAB3027
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MRP, ABCC, GS-X, MRP1, ABC29, DFNA77, MRP1/ABCC1
Positive Sample:
A-549, Mouse brain, Rat brain
Cellular Localization:
Cell Membrane, Multi-Pass Membrane Protein.
Calculated MW:
172kDa
Observed MW:
170-220kDa
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra-and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This full transporter is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a multispecific organic anion transporter, with oxidized glutatione, cysteinyl leukotrienes, and activated aflatoxin B1 as substrates. This protein also transports glucuronides and sulfate conjugates of steroid hormones and bile salts. Alternatively spliced variants of this gene have been described but their full-length nature is unknown.
Purification Method
Affinity purification
Gene ID
4363
RRID
AB_2764833
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MRP1/ABCC1 Rabbit pAb (CAB3027) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Western blot analysis of lysates from A-549 cells, using MRP1/ABCC1 Rabbit pAb (CAB3027) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
