The ADAM15 Antibody (CAB7756) is a high-quality antibody developed for reliable detection and analysis of target proteins. ADAM15, a member of the ADAM family of proteins, is involved in cell adhesion, migration, and signaling pathways. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it an excellent choice for Western blot applications.With its ability to specifically bind to ADAM15 protein, this polyclonal antibody allows for the detection and analysis of ADAM15 in different cell types. Its versatility makes it an ideal reagent for researchers studying cell biology, cancer biology, and signal transduction pathways.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ADAM15 Antibody
SKU:
CAB7756
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MDC15, ADAM15
Positive Sample:
Mouse heart, Mouse kidney, Mouse brain
Cellular Localization:
Cell Junction, Cell Projection, Cytoplasmic Vesicle, Endomembrane System, Single-Pass Type I Membrane Protein, Acrosome, Adherens Junction, Cilium, Flagellum, Secretory Vesicle.
Calculated MW:
93kDa
Observed MW:
93kDa
The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as a EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.
Purification Method
Affinity purification
Gene ID
8751
RRID
AB_2768243
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ADAM15 Rabbit pAb (CAB7756) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using ADAM15 Rabbit pAb (CAB7756) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
