The Placental alkaline phosphatase (PLAP) Polyclonal Antibody (CAB21424) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, created using rabbit immunization techniques, exhibits strong reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the PLAP enzyme, researchers can easily detect and analyze its expression in a variety of cell types.PLAP is a marker often used in diagnosing certain types of cancer, such as seminomas and ovarian germ cell tumors. Its unique presence in placental tissue and tumors makes it a versatile target for studies in oncology and reproductive biology.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human samples.
The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. One of the main sources of this enzyme is the liver, and thus, it's one of several indicators of liver injury in different clinical conditions. In pregnant women, this protein is primarily expressed in placental and endometrial tissue, however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells.
Purification Method
Affinity purification
Gene ID
250
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using Placental alkaline phosphatase (PLAP) Rabbit pAb (CAB21424) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg Placental alkaline phosphatase (PLAP) antibody (CAB21424). Western blot was performed from the immunoprecipitate using Placental alkaline phosphatase (PLAP) antibody (CAB21424) at a dilution of 1:1000.
