The ANAPC4 Polyclonal Antibody (PAC018570) is a valuable tool for researchers studying the ANAPC4 protein, a crucial component of the anaphase-promoting complex (APC). This antibody is produced in rabbits and is highly specific to human samples, making it ideal for use in Western blot applications. The ANAPC4 protein is involved in regulating the cell cycle by targeting specific proteins for degradation, ultimately controlling cell division. Dysregulation of the APC complex, including ANAPC4, has been linked to various diseases, particularly cancer. By using the PAC018570 antibody, researchers can investigate the expression and function of ANAPC4 in different cell types, providing valuable insights into cell cycle regulation and potential therapeutic targets for cancer treatment.
The PAC018570 antibody offers high sensitivity and specificity in detecting ANAPC4 protein levels, allowing researchers to accurately analyze its role in cell division and disease progression. Its use in studying the APC complex provides a deeper understanding of cellular processes and opens up avenues for developing targeted therapies to treat APC-related disorders.
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using PACO18570(ANAPC4 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200).
Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-3: HepG2 cells, 231 cells, hela cells, Primary antibody: PACO18570(ANAPC4 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute.
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO18570(ANAPC4 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans.
Synonyms:
anaphase promoting complex subunit 4
UniProt Protein Function:
APC4: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Belongs to the APC4 family. 2 isoforms of the human protein are produced by alternative splicing.
Molecular Function:protein binding; ubiquitin-protein ligase activity; protein phosphatase binding
Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle
NCBI Summary:
A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The exact function of this gene product is not known. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]
