The Anti-Acetyl Histone H4 (K5, K8, K12, K16) Antibody (CAB20764) offered by AssayGenie is a high-quality polyclonal antibody designed for research involving histone H4 acetylation at lysine residues 5, 8, 12, and 16. Acetylation of histone proteins plays a crucial role in chromatin structure and gene regulation, making this antibody a valuable tool for studying epigenetics and gene expression.Raised in rabbits, this antibody is highly reactive with human samples and has been validated for use in Western blot applications. It specifically binds to the acetylated histone H4 protein at these specific lysine residues, enabling researchers to detect and analyze histone acetylation patterns in various cell types.
Acetylation of histone H4 at lysine 5, 8, 12, and 16 is associated with transcriptional activation and plays a role in chromatin remodeling. Dysregulation of histone acetylation has been implicated in various diseases, including cancer and neurological disorders. By using this antibody, researchers can gain insights into the role of histone acetylation in disease development and potentially identify new therapeutic targets.Overall, the Anti-Acetyl Histone H4 (K5, K8, K12, K16) Antibody (CAB20764) is a valuable tool for researchers interested in studying epigenetic modifications and
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Purification Method:
Affinity purification
Gene ID:
8359
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Acetyl-Histone H4-K5/K8/K12/K16 Rabbit pAb (CAB20764) at 1:500 dilution.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
