The ACHA4 Monoclonal Antibody (CAB0350) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the ACHA4 protein, this antibody enables researchers to study immune responses and their implications in various cell types.ACHA4, also known as a cell surface molecule with immunomodulatory properties, is critical for maintaining immune homeostasis by regulating inflammatory responses and inhibiting allergic reactions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ACHA4 Monoclonal Antibody
SKU:
CAB0350
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1822
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
PEAD GQAA GALA SRNT HSAE LPPP DQPS PCKC TCKK EPSS VSPS ATVK TRST KAPP PHLP LSPA LTRA VEGV QYIA DHLK AEDT DFSV KEDW KYVA MVID R
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:6000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
EBN, BFNC, EBN1, NACHR, NACRA4, NACHRA4, ACHA4
Positive Sample:
SH-SY5Y, U-251MG, C6, Mouse liver, Mouse brain, Rat testis
This gene encodes a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in this gene cause nocturnal frontal lobe epilepsy type 1. Polymorphisms in this gene that provide protection against nicotine addiction have been described. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
1137
RRID
AB_2861459
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ACHA4 Rabbit mAb (CAB0350) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
