The Cardiac Troponin C Monoclonal Antibody (CAB3816) is a high-quality antibody developed for reliable detection and analysis of target proteins. Troponin is a central regulatory protein of striated muscle contraction, and together with tropomyosin, is located on the actin filament. Troponin consists of 3 subunits: TnI, which is the inhibitor of actomyosin ATPase; TnT, which contains the binding site for tropomyosin; and TnC, the protein encoded by this gene. The binding of calcium to TnC abolishes the inhibitory action of TnI, thus allowing the interaction of actin with myosin, the hydrolysis of ATP, and the generation of tension. Mutations in this gene are associated with cardiomyopathy dilated type 1Z. RRID Gene ID 7134 Swiss Prot Synonym TNC; TN-C; TNNC; CMD1Z; CMH13; TNNC1
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
Cardiac Troponin C Monoclonal Antibody
SKU:
CAB3816
Size:
100μL, 20μL
Reactivity:
Mouse, Rat
Clone Number:
ARC0840
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TNC, TN-C, TNNC, CMD1Z, CMH13, TNNC1
Positive Sample:
Mouse heart, Rat heart
Cellular Localization:
Cytosol.
Calculated MW:
18kDa
Observed MW:
18kDa
Troponin is a central regulatory protein of striated muscle contraction, and together with tropomyosin, is located on the actin filament. Troponin consists of 3 subunits: TnI, which is the inhibitor of actomyosin ATPase; TnT, which contains the binding site for tropomyosin; and TnC, the protein encoded by this gene. The binding of calcium to TnC abolishes the inhibitory action of TnI, thus allowing the interaction of actin with myosin, the hydrolysis of ATP, and the generation of tension. Mutations in this gene are associated with cardiomyopathy dilated type 1Z. RRID Gene ID 7134 Swiss Prot Synonym TNC; TN-C; TNNC; CMD1Z; CMH13; TNNC1
Purification Method:
Affinity purification
Gene ID:
7134
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using TNNC1 Rabbit mAb (CAB3816) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
