The eIF3e Monoclonal Antibody (CAB3431) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables protein N-terminus binding activity. Contributes to translation initiation factor activity. Involved in positive regulation of mRNA binding activity; regulation of gene expression; and translational initiation. Located in cytosol and nucleus. Part of eukaryotic translation initiation factor 3 complex. Colocalizes with PML body.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
eIF3e Monoclonal Antibody
SKU:
CAB3431
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1997
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
INT6, EIF3S6, EIF3-P48, eIF3-p46, eIF3e
Positive Sample:
HeLa, 293T, Jurkat, Mouse lung, Mouse testis, Rat lung, Rat testis
Cellular Localization:
Cytoplasm, Nucleus, Pml Body.
Calculated MW:
52kDa
Observed MW:
52kDa
Enables protein N-terminus binding activity. Contributes to translation initiation factor activity. Involved in positive regulation of mRNA binding activity; regulation of gene expression; and translational initiation. Located in cytosol and nucleus. Part of eukaryotic translation initiation factor 3 complex. Colocalizes with PML body.
Purification Method
Affinity purification
Gene ID
3646
RRID
AB_2863057
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using eIF3e Rabbit mAb (CAB3431) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Mouse testis using eIF3e Rabbit mAb (CAB3431) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
