The Gemin 2 Monoclonal Antibody (CAB19254) is a high-quality antibody developed for reliable detection and analysis of target proteins. Gemin-2 is a critical component of the SMN complex, which is essential for the assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs). This antibody, raised in rabbits, exhibits high specificity and sensitivity towards human samples and is validated for use in various applications, including Western blotting and immunofluorescence.Gemin-2 is involved in the assembly and maturation of snRNPs, which play a crucial role in pre-mRNA splicing. Dysregulation of splicing mechanisms has been implicated in various diseases, including spinal muscular atrophy (SMA) and certain types of cancer.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Gemin 2 Monoclonal Antibody
SKU:
CAB19254
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2419
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SIP1, SIP1-delta, Gemin 2
Positive Sample:
U-2 OS, HeLa, MCF7, Mouse testis, Mouse thymus, Rat testis, Rat pancreas
Cellular Localization:
Cytoplasm, Nucleus, Gem.
Calculated MW:
32kDa
Observed MW:
32kDa
This gene encodes one of the proteins found in the SMN complex, which consists of several gemin proteins and the protein known as the survival of motor neuron protein. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal snRNPs and for pre-mRNA splicing. This protein interacts directly with the survival of motor neuron protein and it is required for formation of the SMN complex. A knockout mouse targeting the mouse homolog of this gene exhibited disrupted snRNP assembly and motor neuron degeneration.
Purification Method
Affinity purification
Gene ID
8487
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Gemin 2 Rabbit mAb (CAB19254) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of various lysates using Gemin 2 Rabbit mAb (CAB19254) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
