The HGD Monoclonal Antibody (CAB1400) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes the enzyme homogentisate 1,2 dioxygenase. This enzyme is involved in the catabolism of the amino acids tyrosine and phenylalanine. Mutations in this gene are the cause of the autosomal recessive metabolism disorder alkaptonuria. RRID Gene ID 3081 Swiss Prot Synonym AKU; HGO; HGD
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
HGD Monoclonal Antibody
SKU:
CAB1400
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
ARC2561
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AKU, HGO, HGD
Positive Sample:
HT-29, HepG2
Cellular Localization:
Cytosol, Extracellular Exosome.
Calculated MW:
50kDa
Observed MW:
50kDa
This gene encodes the enzyme homogentisate 1,2 dioxygenase. This enzyme is involved in the catabolism of the amino acids tyrosine and phenylalanine. Mutations in this gene are the cause of the autosomal recessive metabolism disorder alkaptonuria. RRID Gene ID 3081 Swiss Prot Synonym AKU; HGO; HGD
Purification Method:
Affinity purification
Gene ID:
3081
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HGD Rabbit mAb (CAB1400) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
