![Anti-Human CD25 (IL-2Rα) [7G7B6] In Vivo Antibody - Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/1280x1280/products/214346/569414/anti-human-cd25-il-2ra-7g7b6-in-vivo-antibody-low-endotoxin__04651.1772039560.jpg?c=2?imbypass=on "Anti-Human CD25 (IL-2Rα) [7G7B6] In Vivo Antibody - Low Endotoxin")
Description
Anti-Human CD25 (IL-2Rα) [7G7B6] In Vivo Antibody - Low Endotoxin
Introducing the Anti-Human CD25 [IL-2R] 7G7B6 In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the CD25 protein, a component of the interleukin-2 receptor (IL-2R), which is critical for the function and regulation of the immune system, making it an ideal choice for immunological research and related fields. With a mouse IgG1 isotype, it ensures high purity and low endotoxin levels (<1.0 EU/mg), making it perfect for ELISA, flow cytometry, immunohistochemistry, and other assays.
Available in various sizes, this antibody is formulated in phosphate-buffered saline for enhanced stability and efficacy. Enhance your research with this precise and adaptable antibody. CD25 is expressed on the surface of activated T and B cells, regulatory T cells, and a subset of early hematopoietic progenitors. It plays a pivotal role in the immune system by binding interleukin-2 (IL-2) and facilitating the activation and proliferation of T-cells, thus contributing to the immune response.
| Product Name: | Anti-Human CD25 (IL-2Rα) [7G7B6] In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0011 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | 7G7B6 |
| Protein: | CD25 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | IL-2Rα, CD25, Ly-43, p55, Tac |
| Isotype: | Mouse IgG2a |
| Reactivity: | Human |
| Immunogen: | Recombinant human peripheral blood mononuclear cells (PBMCs) |
| Applications: | ELISA, FC, IF Staining, IP, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | ELISA, FC, IF Staining, IP, WB |
| Recommended Usage: | ELISA FC WB |
| Reactivity: | Human |
| Host Species: | Mouse |
| Specificity: | Clone 7G7B6 reacts with human IL-2Rα. |
| Antigen Distribution: | IL-2Rα is expressed on activated mature T and B lymphocytes, during early stages of thymocytes development, pre-B cells, and in activated CD4+ memory T-lymphocytes. |
| Immunogen: | Recombinant human peripheral blood mononuclear cells (PBMCs) |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CD25 (IL-2Rα) is the 55 kDa subunit of the interleukin 2 receptor alpha chain which has been shown to play roles in lymphocyte differentiation, activation, and proliferation. IL-2Rα is a transmembrane glycoprotein comprised of a heavily glycosylated extracellular domain, a transmembrane region and a short cytoplasmic tail. IL-2Rα heterodimerizes with IL-2Rβ (CD122) and IL-2Rγ (CD132) and forms the IL-2 receptor complex. IL-2Rα by itself binds IL-2 with relatively low affinity, while the IL-2 receptor complex binds IL-2 with high affinity. IL-2Rα mutations cause severe immunodeficiency.
| Technical Datasheet: | View |
| Protein: | CD25 |
| Research Area: | Immunology |
![Anti-Human CD25 (IL-2Rα) [7G7B6] In Vivo Antibody - Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/608x608/products/214346/569414/anti-human-cd25-il-2ra-7g7b6-in-vivo-antibody-low-endotoxin__04651.1772039560.jpg?c=2 "Anti-Human CD25 (IL-2Rα) [7G7B6] In Vivo Antibody - Low Endotoxin")
